Genome-scale metabolic reconstructions of Pichia stipitis and Pichia pastoris and in-silico evaluation of their potentials
نویسندگان
چکیده
منابع مشابه
Genome-scale metabolic reconstruction and in silico analysis of methylotrophic yeast Pichia pastoris for strain improvement
BACKGROUND Pichia pastoris has been recognized as an effective host for recombinant protein production. A number of studies have been reported for improving this expression system. However, its physiology and cellular metabolism still remained largely uncharacterized. Thus, it is highly desirable to establish a systems biotechnological framework, in which a comprehensive in silico model of P. p...
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BACKGROUND Pichia pastoris is one of the most important cell factories for production of industrial enzymes and heterogenous proteins. The genome-scale metabolic model of high quality is crucial for comprehensive understanding of the P. pastoris metabolism. METHODS In this paper, we upgraded P. pastoris genome-scale metabolic model based on the combination of latest genome annotations and lit...
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The conversion of pentoses to ethanol is one of the major barriers of industrializing lignocellulosic ethanol processes. As the most promising native strain for pentoses fermentation, Pichia stipitis has been widely studied for its xylose fermentation. In spite of the abundant experimental evidence regarding ethanol and by-products production under various aeration conditions, the mathematical ...
متن کاملEngineering the Pichia stipitis Genome for Fermentation of Hemicellulose Hydrolysates
Pichia stipitis Pignal (1967) is a hemiascomycetous yeast (Kurtzman, 1990; Melake et al., 1996; Vaughan Martini, 1984), closely related to several yeast en dosymbionts of passalid beetles (Nardi et al., 2006) that inhabit and degrade white-rotted hardwood (Suh et al., 2003, 2004). It seems to be well adapted to this environment because the P. stipitis genomic sequence reveals numerous features...
متن کاملCloning and heterologous expression of Laccase in pichia pastoris and determination some of biochemical properties
Laccase (EC 1.10.3.2) are multi-copper oxidase which catalyze the oxidation aromatic and non- aromatic compounds with electron reduction of molecular oxygen to water. Nucleotide sequence of laccase (accession number : ) was optimized according codon preference of Pichia pastoris. Gene was synthesized and cloned into pPICZalpha A. laccase under control of AOX1 promoter was transformed to P.pasto...
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ژورنال
عنوان ژورنال: BMC Systems Biology
سال: 2012
ISSN: 1752-0509
DOI: 10.1186/1752-0509-6-24